W. David Nes's Analysis of Sterols and Other Biologically Significant PDF
By W. David Nes
Research of Sterols and different Biologically major Steroids offers the basic education for the research of chosen sterols and steroids.
The booklet consists of chapters that overview the spectroscopic and chromatographic houses of convinced sterols and steroids. The textual content additionally teaches the right way to isolate and signify sterols and steroid metabolites of plant, fungal, and bug starting place. Lipoprotein research and the usage of physical-analytical recommendations are likewise provided.
Biochemists, microbiologists, and clinical physiologists will locate the booklet necessary.
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Additional resources for Analysis of Sterols and Other Biologically Significant Steroids
Am. Oil Chem. Soc. 62, 1344—1346. , and Bielefeld, B. (1979). Gas chromatographische trennung unpolarer und oxidier ter sterine. /. Chromatogr. 180, 83-91. , and Grunwald, G. (1986). Comparative study of In vivo stigmasterol biosynthesis in Nicotiana tabacum and Hordeum vulgäre. Phytochemistry 12, 2779—2781. Hunter, I. , Waiden, M. , Bailey, G. , and Heftmann, E. (1979). High pressure liquid chromatography and ultraviolet spectrometry of ketonic C 2 7 sterols. Lipids 14, 687-690. , and Djerassi, C.
And Chaikoff, I. L. (1966). Esterification of cholesterol by rat adrenal gland homogenates and subcellular components. Biochim. Biophys. Ada 116, 543-554. Skipski, G. , Smolowe, A. , Sullivan, R. C, and Barclay, M. (1958). Separation of lipid classes by thin-layer chromatography. Biochim. Biophys. Ada 106, 386—396. Stokke, K. , and Norum, K. R. (1970). Subcellular distribution of acyl-CoA: cholesterol acyltransferase in rat liver cells. Biochim. Biophys. Ada 210, 202-204. , and Katsuki, H. (1979).
DETECTION OF STERYL ESTERS A. Nondestructive Detection Visual detection of bands on a thin layer plate can be done in a variety of ways. Appropriate standards are usually developed in lanes adjacent to the lipid samples and can be used to locate the band of interest. Detection can be achieved in a destructive or nondestruve manner, and this choice is dependent upon the information needed from the sample after the TLC phase. Iodine vapor and ultraviolet light are the two most common nondestructive methods.
Analysis of Sterols and Other Biologically Significant Steroids by W. David Nes